Skip to main content
. 2011 Mar 22;60(4):1134–1145. doi: 10.2337/db09-1323

FIG. 6.

FIG. 6.

The interrelationship between stress-induced ErbB Tyr phosphorylation, p38MAPK activation, and IRS-1 Ser phosphorylation. A: HepG2 cells transfected by nontargeted (N.T) or ErbB1 siRNA for 96 h were treated without or with 5 nmol/L TNF for 30 min at 37°C. ErbB1, ErbB1-pTyr1086, ErbB1-pTyr1173, IRS-1-pSer312, IRS-1-pSer616, IRS-1-pSer636, IRS-1, p-p38, and p38 in total cell extract proteins were assessed using Western blot analysis. B: Fao cells, grown at various cell densities for 48 h, were incubated without or with 50 ng/mL AN for 30 min at 37°C. Particulate fractions were analyzed by immunoblot analysis using a mixture of anti-ErbB2 and anti-ErbB3 antibodies or anti-pTyr antibody. Cytosolic fractions were analyzed by immunoblot analysis using antibodies against IRS-1-pSer307, IRS-1-pSer612, IRS-1-pSer632, IRS-1, p-p38, p38, MKK3/6 pSer189/207, and MKK3. Each panel contains a representative blot of three to five independent experiments.