FIG. 6.

DFS protected against SL-induced adipose tissue inflammation by infiltration of M1 macrophages and CD8⁺ T-cells. A: Number of F4/80⁺ cells as a proportion of the cells in the SVF of epididymal fat from the mice as determined by flow cytometry (n = 5). B: Number of CD11c⁺ cells as a proportion of the F4/80⁺ cells in the SVF of epididymal fat from the groups of mice indicated as determined by flow cytometry (n = 5). C: Flow cytometric analysis of CD11c⁺ cells among F4/80⁺ SVF cells from the epididymal fat of Gck^+/− mice. D and E: Flow cytometric analysis of the CD4⁺ subset and CD8⁺ subset of CD3⁺ T-cells in the epididymal fat of the groups of mice indicated (n = 5). F: Assessment of the level of expression of the mRNAs indicated in epididymal fat as determined by real-time quantitative RT-PCR and normalization to the β-actin mRNA level (n = 5). G and H: Serum TNF-α and MCP-1 levels (n = 6–8). Experiments were performed on wild-type (WT) and Gck^+/− mice after 20 weeks on the SL or the SL-plus-DFS diet. *P < 0.05.