Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Mar 22;60(4):1314–1323. doi: 10.2337/db10-1557

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

PMC Copyright notice

FIG. 1. — MiRNA and VEGF alteration in the retina in diabetes. Quantitative RT-PCR (A) and ELISA (B) analysis from nondiabetic control and diabetic rat retinal tissue samples after 1 month of follow-up show increased levels of VEGF mRNA and protein. C: Volcano plot shows miRNA alteration in control vs. diabetic (treated) rat retina after 1 month. Circles to the left of −1 difference line and to the right of the 1 difference line are considered to have a fold-change >2× (x-axis is the log2 of the fold-change between two groups). The arrow indicates the location of miR-200b. (Custom analysis using Asuragen miRNA system. Some of the highly expressed miRNAs represent putative miRNA sites, which require biological characterization). Data array were validated with qRT-PCR analyses, which confirmed downregulation of miR-200b in the retina of diabetic rats compared with the nondiabetic controls (D) and no significant changes in the expression of miR-429 (E), miR-200a (F), and miR-200c (G). The miRNA data are expressed as a ratio to RNU6B (U6); mRNA levels are expressed as a ratio to 18S RNA, and normalized to controls. The error bars show the SEM. *Significantly different from the other group. (A high-quality color representation of this figure is available in the online issue.)