Fig. 2.

TNBS-produced visceral hypersensitivity is significantly attenuated in GFRα3 KO mice. Intracolonic treatment with TNBS (B) but not saline (A) significantly increased visceromotor responses to distension [saline, n = 7, overall F(4,24) = 3.65; Holm-Sidak post hoc test, t = 1.78, P > 0.05; TNBS, n = 7, F(4,24) = 9.10, P < 0.001]. Data are expressed as means ± SE of responses normalized to the pretreatment baseline response to 60 mmHg. C: AUC derived from the data presented in A and B. Visceral hypersensitivity produced by TNBS in GFRα3 KO mice significantly differed in magnitude across days after treatment [F(1,48) = 10.43, P = 0.007]. D: comparison of TNBS-induced stimulus-response curves (AUC) between C57BL/6 (from Fig. 1D) and GFRα3 KO mice. TNBS induced significantly greater visceral hypersensitivity in C57BL/6 mice than in GFRα3 KO mice [F(1,44) = 4.96, P = 0.048].