Fig. 6.

Effect of chronic alcohol feeding on activity of the SLC5A6 5′-regulatory region in kidneys of transgenic mice carrying full-length promoter construct. A: SLC5A6 5′-regulatory region activity in kidneys of alcohol-fed transgenic mice and their pair-fed controls kidney as determined by a luminometer and presented in luciferase light units. Mice carrying the human SLC5A6 5′-regulatory region (P1P2) were used, as described in materials and methods. B: real-time PCR was performed on RNA isolated from alcohol-fed and pair-fed control mice kidney cortex using mouse SMVT gene-specific primers. Values are means ± SE of 3 separate samples from 3 mice. *P < 0.05, **P < 0.01.