Fig. 3.
Effect of inhibition of cyclooxygenase or nitric oxide synthase on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells. Cells were preincubated with vehicle, 1 mM NG-nitro-l-arginine methyl ester (l-NAME), or 1 μM indomethacin for 30 min, followed by exposure to shear stress at 2 dyne/cm2 for 2 h in the presence of the same agents and then determination of ET-1/GAPDH mRNA levels; n = 6–8 each data point. *P < 0.05 vs. cells treated identically with the same agents, but not exposed to flow (control).