Skip to main content
. 2010 Dec 22;300(3):F650–F656. doi: 10.1152/ajprenal.00530.2010

Fig. 4.

Fig. 4.

Effect of modulation of Ca2+ pathways on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells. Cells were preincubated with vehicle, HBSS without Ca2+, 50 μM BAPTA (to chelate intracellular Ca2+), 100 μM nifedipine, or 10 μM W-7 (CaM inhibition) for 30 min, followed by exposure to shear stress at 2 dyne/cm2 for 2 h in the presence of the same agents/media and then determination of ET-1/GAPDH mRNA levels; n = 6–8 each data point. *P < 0.05 vs. cells treated identically with the same agents, but not exposed to control.