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. 2010 Dec 22;300(3):F650–F656. doi: 10.1152/ajprenal.00530.2010

Fig. 5.

Fig. 5.

Effect of modulation of protein kinase C (PKC) and phospholipase C (PLC) on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells. For phorbol 12-myristate 13-acetate (PMA) studies, cells were preincubated with vehicle or 50 ng/ml PMA for 2 h. Otherwise, cells were incubated with vehicle, 100 nM calphostin C (PKC inhibitor), or 2 μM U73122 (PLC inhibitor) for 30 min. After preincubations, cells were exposed to shear stress at 2 dyne/cm2 for 2 h in the presence of the same agents and then determination of ET-1/GAPDH mRNA levels; n = 6–8 each data point. *P < 0.05 vs. cells treated identically with the same agents, but not exposed to flow (control).