Fig. 3.

Localization of renin-expressing cells by immunoreactivity in kidney sections of Cx45^fl/fl and renin-Cre:Cx45^fl/fl mice during a normal-salt diet or after a low-salt diet combined with enalapril. Immunohistochemistry is shown of renin (green) and α-smooth muscle actin (SMA; red) in the control (a, normal-salt diet; b, low-salt diet/enalapril) and renin-Cre:Cx45^fl/fl mice (c, normal-salt diet; d, low-salt diet/enalapril). Bar = 50 μm. Asterisks indicate the glomeruli, and arrowheads indicate renin-producing cells. Five kidneys of each genotype have been analyzed. There is no difference in the number and the localization of the renin-expressing cells between the 2 genotypes.