TABLE 1.
Properties of OleA compared with homologous proteins in the thiolase superfamily
| Protein (organism)a | Accession number | Sequence identityb | Calculated Mr | Calculated pI | Cellular function | Claisen mechanism | Sequence signaturec |
|---|---|---|---|---|---|---|---|
| % | |||||||
| OleA (X. campestris) | NP_635607 | 100 | 36,629 | 5.6 | Alkene biosynthesis | Proposed | NACLAFING |
| Non-decarboxylative | |||||||
| OleA (M. luteus) (11) | YP_002957382.1 | 38 | 36,653 | 4.8 | Alkene biosynthesis | Proposed decarboxylative | NACLGFVNG |
| Thiolase (Zoogloea ramigera) (23) | AAA27706.1 | 19 | 40,416 | 5.9 | PHB biosynthesis | Non-decarboxylative | QLCGSGLRA |
| HMG-CoA synthase (Homo sapiens) (26) | 1XPL_A | 16 | 43,204 | 5.0 | Mevalonate pathway | Non-decarboxylative | EACYAATPA |
| FabH (E. coli) (25) | 1EBL_A | 24 | 33,523 | 5.1 | Fatty acid biosynthesis | Decarboxylative | AACAGFTYA |
| Mycobacterium Pks13d (24) | CAA17864 | 19 | 44,122 | 5.2 | Mycolic acid biosynthesis | Decarboxylative | TACSSSLVA |
a Numbers in this column represent references from which the data in the table were obtained.
b Via Needleman-Wunch and BLAST algorithms and comparison with OleA from X. campestris.
c Amino acid sequence surrounding the active site cysteine conserved in thiolase superfamily proteins.
d Alignment to keto-acyl synthase domain only, as defined by NCBI.