FIGURE 4.

Effect of neutralizing antibodies on ET-1-induced MCP-1 and IL-6 protein secretion. Serum-starved mesangial cells were preincubated for 1 h with neutralizing monoclonal antibodies against IL-6 (0.1 μg/ml; clone 6708) (A) and MCP-1 (5 μg/ml; clone 24822) (B) before the addition of 100 nm ET-1 at time 0. MCP-1 and IL-6 peptide secretion was measured by ELISA. To control for nonspecific effects, an irrelevant monoclonal antibody (No IgG; 5 μg/ml) was added. Data are means ± S.D. for three experiments performed in duplicate, and there was not a statistically significant difference in MCP-1 or IL-6 secretion at any time point.