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ET-1/ETA receptor signaling elevates collagen secretion in mesangial cells. Quiescent mesangial cells were pretreated for 3 h with BQ-123 (250 nm) and BQ-788 (1.0 μm) before the addition of 100 nm ET-1 at time 0 and measurement of total collagen in the cell monolayer at the times indicated. Data are means ± S.D. for three independent experiments performed in duplicate. **, p < 0.01 versus cells treated with vehicle or with ET-1 and BQ-123 at the same time point.
