Skip to main content
. 2011 Jan 28;286(13):11056–11066. doi: 10.1074/jbc.M110.204081

FIGURE 2.

FIGURE 2.

IL-6-directed migration activity of human chondrosarcoma cells involves up-regulation of MMP-13. A, JJ012 cells were incubated with IL-6 for 24 h, and the mRNA levels of MMP-1, -2, -3, -9, and -13 were determined by qPCR. B, JJ012 cells were incubated with IL-6 for the indicated time periods. The cultured medium and cell lysates were then collected. The protein level of MMP-13 in cell lysates and the enzyme activity of MMP-13 in the supernatant were examined by Western blotting and zymography. C, cells were transfected with MMP-13 siRNA for 24 h followed by stimulation with IL-6, and in vitro migration was measured after 24 h. D and E, JJ012 cells were pretreated with anti-IL-6R Ab for 30 min followed by stimulation with IL-6 for 24 h. The mRNA level of MMP-13 in cell lysates and the enzyme activity of MMP-13 in the supernatant were examined by qPCR and zymography. F, JJ012 cells were transfected with IL-6 or control siRNA for 24 h followed by stimulation with IL-1β, and mRNA expression of MMP-13 was examined by qPCR after 24 h. Results are expressed as means ± S.E. *, p < 0.05 compared with the control; #, p < 0.05 compared with the IL-6-treated group.