FIGURE 10.

Infusion with AICAR increases the activation of AMPK in vivo. C57BL/6J mice fed a HFD were treated with AICAR (200 mg/kg/day, n = 8) or vehicle (0.9% NaCl, n = 8) in the absence or presence of compound C (n = 8, 20 mg/kg/day) for 4 weeks. A, AMPK Thr-172 phosphorylation in liver tissue extracts was analyzed by Western blot. The data represent results of three independent experiments. B, AMPK activity assays with the use of SAMS peptide as a substrate were also performed on aorta extracts. Data represent the means ± S.E. n = 6 per group. *, p < 0.05.