FIGURE 7.

AMPK promotes FOXO1 deacetylation. A, HepG2 cells were treated with AICAR for the times indicated. Then total protein lysates were used for immunoprecipitation (IP) of FOXO1, and FOXO1 acetylation levels were measured by Western blot (IB). B, relative band intensities were quantified. Data represent the means ± S.E. *, p < 0.05; or **, p < 0.01 versus 0 h. HepG2 cells were infected with adenoviruses encoding constitutively active (CA) or dominant-negative (DN) forms of AMPK (C) or transfected with either control (Ctr) or SIRT1 siRNA (Si) (D). Next, cells were treated with vehicle or AICAR (1 mm) for 8 h. Then, the acetyl-lysine level was determined in FOXO1 immunoprecipitates. E, the phosphorylation of Thr-172 of AMPK in the cellular extracts was measured by Western blot. The data represent results of three independent experiments.