FIGURE 5.

Activation of AMPK and decreased malonyl-CoA levels in hearts of MHC-PDK4 mice. A, top, representative Western blot analyses were conducted on lysates of heart ventricles of female mice of indicated genotypes for phosphorylated (P) and total AMPK and acetyl-CoA carboxylase (ACC) as denoted. Bottom, bars represent the ratio in arbitrary units (AU) of phosphorylated/total levels of AMPK or acetyl-CoA carboxylase as indicated normalized (=1.0) to the NTg (n = 7 per group). *, p < 0.05 compared with NTg. B, bars represent the mean (±S.E.) cardiac malonyl-CoA levels as determined from heart ventricles of male mice from indicated genotypes (n = 6–9 per group). *, p < 0.05 compared with NTg.