FIGURE 6.

Induction of PGC-1α gene expression and increased respiratory uncoupling in mitochondria isolated from MHC-PDK4 hearts. A, levels of specific mRNAs based on quantitative real-time-PCR performed on RNA isolated from heart ventricles of male mice from the genotypes indicated (n = 8–10 per group) are shown. Bars represent arbitrary uints (AU) normalized (=1.0) to the NTg. B, mitochondrial respiration rates determined by oxygen consumption (VO₂) performed on mitochondria isolated from ventricle of male NTg and MHC-PDK4 hearts are shown. Mean values (±S.E.) are shown for the basal, ADP-stimulated, and oligomycin-inhibited states using succinate/rotenone as a substrate. The mean respiratory control (RC) ratio (±S.E.) is shown on the right. *, p < 0.05 compared with NTg.