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. 2011 Feb 8;286(13):11163–11169. doi: 10.1074/jbc.M110.212704

FIGURE 4.

FIGURE 4.

Peptidase activity of NukT using ISO or RSO membrane vesicles. Modified His-NukA (3 μm) was incubated with 1 mg/ml NukT membrane. Nukacin ISK-1 produced by cleavage of the leader peptide was detected from its antibacterial activity using the overlay assay method with L. sakei as the indicator strain.