FIGURE 3.

Nucleosome remodeling activities of hINO80 complex and hINO80 complex subassemblies. Nucleosome sliding assays were performed with the indicated complexes as described under “Experimental Procedures.” hINO80 complexes at a relative concentration (rel. conc.) of 4 contain ∼400 fmol of Arp5, equivalent to the amount of material loaded onto the gels shown in Fig. 2, B and C. Percent remodeled nucleosomes is equivalent to the amount of radioactivity in the upper band, corresponding to the centrally positioned remodeled nucleosome, divided by the total amount of radioactivity in the remodeled nucleosome and the prominent lower band, which corresponds to the laterally positioned, starting nucleosome (see lane 15). The faint band at the bottom of the gels is due to a small amount of free DNA in the nucleosome preparation. The data in lanes 1–15, 16–23, and 24–27 are from three separate experiments; quantitative comparisons should be made only within an individual experiment.