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Yeast complementation assay of aromatic amino acids in TMD1. A, a replacement of the hydrophobic amino acid Phe-24 by the hydrophobic amino acid Leu did not rescue yeast growth on selective media. B, substitution of Phe-24 and Tyr-28 by other amino acids with an aromatic side chain rescued yeast growth on selective media. The complementation assay was done as described in Fig. 2.
