TABLE 1.
Quantification of EGFR and HER2 levels in various cell lines using the QIFI kit and anti-EGFR (m225) or anti-HER2 (FSP77) antibodies
The calibration curve was drawn using data obtained from control beads. The antigen density was expressed as the ABC in molecules/cell.
| Cell lines | EGFR ABC | HER2 ABC |
|---|---|---|
| 103 molecules/cell | 103 molecules/cell | |
| NIH/3T3-R1 | 1903 ± 266a | Undetectable |
| NIH/3T3-R2 | Undetectable | 187.2 ± 21.1 |
| NIH/3T3-R1R2 | 2188 ± 239a | 183.9 ± 24.3 |
| BT474 | 10.5 ± 2.1 | 478.0 ± 75.8 |
| A431 | 823.9 ± 46.5a | 35.7 ± 3.1 |
| SKOV-3 | 140.7 ± 10.1 | 329.7 ± 36.2 |
| BXPC-3 | 203.3 ± 14.0 | 10.9 ± 1.7 |
| Capan-1 | 26.7 ± 4.6 | 15.6 ± 2.4 |
| MiaPaCa-2 | 60.8 ± 9.9 | 21.5 ± 3.7 |
| SKBR-3 | 41.6 ± 12.1 | 1766 ± 243a |
a Values with an asterisk were extrapolated from the QIFI kit range, between 2,000 and 518,000 molecules/cell.