TABLE 4.
The KD values of galectin-8-N-domain and the mutants for various oligosaccharides
| Oligosaccharide | Wild-type | R59A | R45A | Q47A |
|---|---|---|---|---|
| m | ||||
| Lac-pNP | 7.4 × 10−5 (1.0)a | 1.0 × 10−4 (0.7) | 1.9 × 10−4 (0.4) | 1.2 × 10−4 (0.6) |
| Siaα2→3Lac (3′-SL) | 2.7 × 10−6 (27) | 4.7 × 10−4 (0.2) | 3.7 × 10−5 (2) | 3.7 × 10−5 (2) |
| Gal-core2-pNP | 5.9 × 10−5 (1.3) | 1.4 × 10−4 (0.5) | 1.7 × 10−4 (0.4) | 1.0 × 10−4 (0.7) |
| Siaα2→3Gal-core2-pNP (3′-Sgal-core2-pNP) | 1.4 × 10−6 (53) | 6.9 × 10−5 (1.1) | 1.7 × 10−5 (4.3) | 1.1 × 10−5 (6.7) |
| LNT | 7.3 × 10−5 (1.0) | 6.5 × 10−5 (0.8) | 3.2 × 10−4 (0.2) | 3.5 × 10−5 (2.1) |
| SO3−→3LNT (3-sulfoLNT) | 1.4 × 10−6 (53) | 7.0 × 10−5 (0.9) | — b | —-b |
a The relative binding abilities in the parenteses were calculated by dividing the KD value between Lac-O-pNP and galectin-8-N-domain at 25 °C by the KD values for the oligosaccharides.
b No binding up to 11 μm.