Table 1.
Reactivities of sera from mice immunized with (Ley)3-peptide-Pam3Cys and (Ley)3-peptide-KLH conjugates with OVCAR-3 ovarian cancer cells analyzed by flow cytometry
| Mice | Immunogen | Ig class tested | Mean fluorescence | % Positive cells |
|---|---|---|---|---|
| Group A | (αLey)3-Pam3Cys (1) | IgM | 446.2 ± 109 | 72.5 ± 4.5 |
| IgG | <10.0 | <5.0 | ||
| Group B | (βLey)3-Pam3Cys (2) | IgM | 518.2 ± 69.7 | 73.7 ± 2.7 |
| IgG | <10.0 | <5.0 | ||
| Group C | (αLey)1-Pam3Cys (3) | IgM | 267.4 ± 20.5 | 57.4 ± 10.8 |
| IgG | <10.0 | <5.0 | ||
| Group D | (αLey)3-Pam3Cys (4) + QS21 | IgM | 170.1 ± 30.0 | 77.8 ± 4.9 |
| IgG | 37.1 ± 6.7 | 49.8 ± 19.0 | ||
| Group E | (αLey)3-KLH (5) + QS21 | IgM | ||
| IgG | 72.6 ± 7.6 | 23.3 ± 14.6 |
Groups A vs. C (P = 0.03) and B vs. C (P = 0.03) were compared by using a two-sample t-test.