FIGURE 3.
Effects of TyrRSMini, TyrRSMini-Mono, and TyrRSMini-Dimer on PMN cell migration activity. a, TyrRSMini-Dimer inhibits WT TyrRSMini-induced PMN cell migration. b, human PMN cell migration was performed using a Transwell permeable support with 5-μm pores as described in the supplemental material. Data from at least four experiments were collected, and mean ± S.D. is shown. c, a schematic diagram illustrating a proposed model for how TyrRSMini mediates and regulates cytokine signaling. In this model, the dissociated monomer form of homodimeric TyrRSMini is the active cytokine, and the dimer is a non-functional antagonist. Dimeric TyrRSMini is labeled with individual monomers shown in lemon and banana, and the ELR motif is labeled in red.