FIGURE 1.
Gross abnormality in the skin of PLA2G10-Tg mice. A, shown is expression of sPLA2-X in the skin of PLA2G10-Tg (X-Tg) and control (Ct) mice at P25, as assessed by RT-PCR. B, gross appearances of X-Tg and control littermates are shown. X-Tg mice were normal at birth (P0), and no gross abnormalities in coat hairs were apparent by P10. X-Tg mice began to lose coat hairs by P15 and were entirely bald over P20–P30. After this hairless period, new hairs reappeared in PLA2G10-Tg mice by P42. After 18 weeks, the fur of X-Tg mice looked matted, lusterless, coarse and ungroomed. C, a dorsal view of the head (whiskers) of control and X-Tg mice at P25 is shown. There were few whiskers on the Tg snout. D, shown is a dorsal view of the wrinkled skin of X-Tg mice at P20. E, PLA2 enzymatic activity in homogenates of skin from control (open columns) and X-Tg (solid columns) mice at each postnatal point is shown. Data are the means ± S.D. (n = 4). F, detection of sPLA2-X protein in skin homogenates by immunoblotting is shown. The homogenates from X-Tg and control mice at the indicated postnatal points were immunoprecipitated with mouse monoclonal anti-human sPLA2-X antibody and immunoblotted with rabbit polyclonal anti-human sPLA2-X antibody. Positions of mature, zymogen, and glycosylated forms of sPLA2-X protein, as evaluated by comparison with a positive control (PC12 cells infected with adenovirus expressing sPLA2-X (22)) (C), are indicated in the right margin.