FIGURE 9.
Increased COX-2 expression and PGE2 synthesis in hair follicles of Pla2g10−/− mice. A, hair follicle and epidermis fractions were isolated by laser-capture microdissection from the skins of Pla2g10+/+ (open columns) and Pla2g10−/− (solid columns) mice (n = 6). PGE2 contents in the isolated hair follicle or epidermis fractions were quantified by enzyme immunoassay. B and C, quantitative RT-PCR of various mRNAs for PGE2 synthesis and its receptor in hair follicles (B) and epidermis (C) is shown. Relative abundance of their transcripts was normalized relative to the constitutive expression of Actb mRNA. Data are the means ± S.E. (n = 6).