TABLE 1.

Steady state and transient kinetic parameters of myosin (M) VI-S1 constructs in the presence of actin at 25 °C

Kinetic parameters	MVI MDins2IQ WT	MVI MDins2IQ L310G	MVI MDins2IQ (Δ-Insert-1)
Steady state parameters
Vmax (s−1)a	6.8 ± 0.2	10.3 ± 0.4	17.7 ± 1.8
KATPase (μm)a	8.3 ± 0.2	8.5 ± 0.3	20.4 ± 3.4
ADP binding to actomyosin VI (k−5′) (μm−1s−1)b	0.27 ± 0.06	0.37 ± 0.08	0.43 ± 0.05
ADP release from actomyosin VI (k+5′) (s−1)b	6.3 ± 0.50	10.2 ± 0.10	18.0 ± 0.7
ATP-induced binding and dissociation from pyrene-actin
K1′k+2′ (μm−1·s−1)c	0.023 ± 0.01	0.28 ± 0.06	0.32 ± 0.04

^a Measured in the NADH coupled assay.

^b Measured with mantADP.

^c Measured with pyrene actin.