TABLE 2.

Steady state and transient kinetic parameters of myosin (M) VI-S1 constructs in the absence of actin at 25 °C

Kinetic parameters	MVI MDins2IQ WT	MVI MDins2IQ L310G	MVI MDins2IQ (Δ-Insert-1)
Steady state
V0 (-actin) (s−1)a	0.03 ± 0.01	0.06 ± 0.02	0.18 ± 0.05
ADP binding to myosin VI (k−5) (μm−1s−1)b	0.68	0.64	0.66
ADP release from myosin VI (k+5) (s−1)b	6.3 ± 0.20	10.5 ± 0.30	2.2 ± 0.01
ATP binding to myosin VI (K1k+2) (μm−1s−1)b	0.15 ± 0.02	1.15 ± 0.12	1.09 ± 0.08

^a Measured in the NADH-coupled assay.

^b Measured with mantATP or mantADP.