TABLE 2.
Phenotypic analysis of TolQ double cysteine mutations
Same legend as Table I except that additional complexes (see supplemental Fig. S4) are indicated in the Complex formation column (di, dimer; (di), weak dimer; in, intramolecular bond; x, multimer).
| Mutation | Colicin activities |
DOC | Complex formation | Phenotype | ||
|---|---|---|---|---|---|---|
| A | E2 | D | ||||
| WT | 4 | 4 | 4 | R | − | B |
| tolQ | R | R | 4 | S | − | R |
| Periplasmic side | ||||||
| L19C/A155C | 4 | 4 | 4 | 3 | − | B |
| L19C/L156C | 4 | 4 | 4 | 3 | − | B |
| L19C/G157C | 4 | 4 | 4 | R | (di) | B |
| L19C/P169C | 3 | 3 | 4 | S | − | C |
| L19C/G170C | 4 | 4 | 4 | 3 | − | B |
| L19C/I171C | 4 | 4 | 4 | R | − | B |
| I20C/A155C | 4 | 4 | 4 | 3 | (di) | B |
| I20C/L156C | 4 | 4 | 4 | 1 | − | C |
| I20C/G157C | 4 | 3 | 4 | S | di | C |
| I20C/P169C | 3 | 3 | 4 | S | − | C |
| I20C/G170C | 4 | 4 | 4 | 2 | − | C |
| I20C/I171C | 4 | 4 | 4 | 3 | − | B |
| M21C/A155C | 4 | 4 | 4 | 3 | − | B |
| M21C/L156C | 4 | 4 | 4 | 3 | − | B |
| M21C/G157C | 4 | 4 | 4 | 1 | di | C |
| M21C/P169C | R | R | 4 | S | − | R |
| M21C/G170C | 4 | 4 | 4 | 1 | − | C |
| M21C/I171C | 4 | 4 | 4 | 3 | − | B |
| A155C/P169C | 3 | 3 | 4 | S | x | C |
| A155C/G170C | 4 | 4 | 4 | S | (di) | C |
| A155C/I171C | 4 | 4 | 4 | 1 | (di) | C |
| L156C/P169C | 3 | 3 | 4 | S | (di) | C |
| L156C/G170C | 2 | 2 | 4 | S | (di) | C |
| L156C/I171C | 4 | 4 | 4 | 1 | (di) | C |
| G157C/P169C | R | R | 4 | S | x | R |
| G157C/G170C | 4 | 4 | 4 | 1 | x | C |
| G157C/I171C | 4 | 4 | 4 | 1 | (di) | C |
| Cytoplasmic side | ||||||
| I35C/P138C | 4 | 4 | 4 | 1 | − | C |
| I35C/Y139C | 4 | 4 | 4 | 1 | − | C |
| I35C/I140C | 4 | 4 | 4 | 2 | − | C |
| I35C/A185C | 4 | 4 | 4 | 1 | − | C |
| I35C/I186C | 4 | 4 | 4 | 1 | − | C |
| I35C/P187C | 3 | 3 | 4 | 1 | − | C |
| I36C/P138C | 4 | 4 | 4 | 2 | in-(di) | C |
| I36C/Y139C | 4 | 4 | 4 | 1 | in-(di) | C |
| I36C/I140C | 4 | 4 | 4 | 3 | in | B |
| I36C/A185C | 4 | 4 | 4 | 1 | − | C |
| I36C/I186C | 3 | 2 | 4 | 1 | − | C |
| I36C/P187C | 4 | 3 | 4 | 2 | − | C |
| Q37C/P138C | 4 | 4 | 4 | 2 | − | C |
| Q37C/Y139C | 3 | 3 | 4 | 1 | (di) | C |
| Q37C/I140C | 4 | 4 | 4 | 2 | (di) | C |
| Q37C/A185C | 4 | 4 | 4 | R | in | B |
| Q37C/I186C | 3 | 2 | 4 | 2 | in-(di) | C |
| Q37C/P187C | 4 | 2 | 4 | 2 | (di) | C |
| P138C/A185C | 3 | 3 | 4 | 2 | − | C |
| P138C/I186C | 4 | 4 | 4 | 1 | − | C |
| P138C/P187C | R | R | 4 | S | − | R |
| Y139C/A185C | 4 | 4 | 4 | 1 | di | C |
| Y139C/I186C | 3 | 2 | 4 | S | di | C |
| Y139C/P187C | 3 | 2 | 4 | 1 | − | C |
| I140C/A185C | 4 | 4 | 4 | 1 | di | C |
| I140C/I186C | 3 | 2 | 4 | S | − | C |
| I140C/P187C | 4 | 3 | 4 | S | − | C |