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. Author manuscript; available in PMC: 2011 Aug 3.
Published in final edited form as: Nature. 2011 Feb 3;470(7332):124–128. doi: 10.1038/nature09658

Figure 2. MMSET is required for H4K20me2/3 and 53BP1 accumulation at DSBs.

Figure 2

a, Quantitative PCR analysis of ChIP samples from HeLa-DR-GFP cells transfected with control or MMSET shRNA, where the Y axis represents the relative enrichment of the indicated proteins compared to the IgG control (± s.e.m., n=3). b, EtBr staining of ChIP samples from 2a analyzed by PCR, where input demonstrates equal loading of DNA for PCR. c–d, IF of HCT116 cells transfected with the indicated siRNA or shRNA, irradiated (5Gy), and stained with indicated antibodies. e, Phosphorylation of CHK1/2 in the cell lysates from 2c analyzed by immunoblotting.