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. Author manuscript; available in PMC: 2011 Aug 3.
Published in final edited form as: Nature. 2011 Feb 3;470(7332):124–128. doi: 10.1038/nature09658

Figure 4. Phosphorylation of MMSET is important for H4K20 methylation, 53BP1 recruitment and DNA damage response.

Figure 4

a, HeLa DR-GFP cells were transfected with the indicated constructs, H4K20 methylation and MMSET recruitment was analyzed by PCR of ChIP samples. b–c, HCT116 cells transfected with indicated constructs were irradiated, and 10 min later, stained with indicated antibodies. d, Radiation sensitivity of cells from Figure 4c was determined by colony formation (±s.e.m., n=3). E, Model demonstrating how the MDC1-MMSET pathway regulates DNA damage-induced histone H4 Lysine 20 methylation and 53BP1 foci formation.