Fig. 2.

Selective inhibitors of protein farnesylation or geranylgeranylation markedly attenuate glucose-induced ROS generation in INS 832/13 cells and normal rodent islets. INS 832/13 cells (A) or normal rat islets (B) were incubated overnight in the absence or presence of FTI-277 (5 μM; left) or GGTI-2147 (10 μM; right), followed by stimulation with either LG (2.5 mM) or HG (20 mM) for 1 h. ROS generated was quantified as DCF fluorescence and expressed as AU. Values are means ± SE from three independent experiments done in triplicates (in INS 832/13 cells) and in duplicates (in islets) in each case. *P < 0.05 vs. LG alone. **P < 0.05 vs. HG alone.