Fig. 3.

Selective inhibitors of protein prenylation inhibit ROS generation induced by a mixture of mitochondrial (mito) fuels in INS 832/13 cells. INS 832/13 cells were incubated overnight in the presence or absence of FTI-277 (5 μM; A) and GGTI-2147 (10 μM; B), followed by stimulation with LG (2.5 mM) or a mixture of mito fuels [monomethyl succinate (MMS) = 20 mM and α-ketoisocaproic acid (KIC) = 5 mM] for 1 h in continuous presence or absence of inhibitors. ROS generated was quantified as DCF fluorescence and expressed as AU. Values are means ± SE from three independent experiments done in triplicates in each case. *P < 0.05 vs. glucose alone. **P < 0.05 vs. mito fuels alone.