Fig. 5.

Pertussis toxin (Ptx) pretreatment attenuates glucose-induced ROS generation in INS 832/13 cells or normal rat islets. Untreated or Ptx-treated (100 ng/ml) INS 832/13 cells (A) or normal rat islets (B) were stimulated with either LG (2.5 mM) or HG (20 mM) for 1 h. ROS generated was quantified as DCF fluorescence and expressed as AU. Values are means ± SE from three independent experiments done in triplicates (in INS 832/13 cell) and in duplicates (in islets) in each case. *P < 0.05 vs. LG alone. **P < 0.05 vs. HG alone.