Fig. 1.

Doxycycline (Dox) treatment of A-C/EBP mice. A: scheme for 3 different Dox treatment regimens. For groups A and C, the pups were fed Dox before birth through the mother and feeding continued for 4 mo after birth. Each group consists of mice expressing A-C/EBP (A-C/EBP+) and those not expressing A-C/EBP (A-C/EBP−). B: quantitative RT-PCR (qRT-PCR) analysis of A-C/EBP expression in lungs of 3 groups of mice as described in A, as well as TetO-A-C/EBP monogenic mice fed normal chow and Dox diet for 4 mo. Each symbol corresponds to a mouse. C: expression level of SCGB3A2 and SCGB1A1 was analyzed by qRT-PCR. The relative expression levels are expressed based on the mean values (±SD) of wild-type mice as 1. **P < 0.01 by Student's t-test.