Fig. 1.

Generation of transgenic mice overexpressing type 5 adenylyl cyclase (AC5). A: genomic PCR for the transgene using a primer from rat AC5 and one from SV40 of the transgene. B: mRNA expression from RT-PCR using rat-specific AC5 primers shows rat AC5 mRNA expression in airway smooth muscle (ASM) exclusively from mice that express the SMP8-AC5 transgene. C: AC5 protein expression in mice expressing the SMP8-AC5 transgene is increased twofold in ASM compared with that in nontransgenic (NTG) mice. Shown are results from two NTG controls and three different lines (F2) of AC5 transgenics. D: forskolin (FK)-mediated airway relaxation from acetylcholine (ACh)-constricted rings is greater in AC5 mice. Values are means ± SE. Results are from experiments with 4 NTG and 4 AC5 transgenic mouse tracheas. *P < 0.05 vs. NTG.