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. 2010 Dec 24;300(3):L422–L429. doi: 10.1152/ajplung.00166.2010

Fig. 3.

Fig. 3.

Double-stranded RNA-dependent protein kinase (PKR) is activated by hyperoxia and induces downstream CHOP expression. A: representative immunoblots of whole cell lysates for phosphorylated PKR (p-PKR) and total PKR from MLE-12 cells exposed to 95% O2-5% CO2 for up to 24 h or 5 μM tunicamycin for 24 h. B–D: PKR, ATF4, and CHOP mRNA expression normalized to HPRT1 in MLE-12 cells exposed to transfection reagent alone (untreated), PKR small interfering RNA (siRNA), or control (cyclophilin B) siRNA (25 nM for 72 h). Cells were exposed to normoxia (21% O2) or hyperoxia (95% O2) for the final 24 h. Data summarize 3 independent experiments. *P < 0.05.