Fig. 2.
Downregulation of SERT using small interfering RNA (siRNA) attenuates PDGF-induced PASMC proliferation. HPASMCs were transfected with SERT siRNA and nontargeted siRNA and then evaluated for cell proliferation by a [3H]thymidine incorporation assay. The expressions of SERT and GAPDH were detected by Western blotting by using SERT and GAPDH antibody, respectively. Bar graphs shown are means ± SD, n = 4. *Significant difference from cells without PDGF treatment correspondingly (P < 0.05). #Significant difference from cells transfected with control/nontargeting (Con) siRNA plus PDGF treatment (P < 0.05). Non siRNA, no siRNA transfection.