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. 2010 Dec 24;300(3):L486–L497. doi: 10.1152/ajplung.00237.2010

Fig. 4.

Fig. 4.

SERT participates in p38 MAPK pathway in PDGF-induced PASMC proliferation. A: PDGF-induced p38 phosphorylation is attenuated by SERT and PDGFR inhibitors. Quiescent HPASMCs and BPASMCs were treated with 10 ng/ml PDGF for 5 min in the absence or presence of 10 μM fluoxetine, 10 μM imipramine, 1 μM imatinib, or 10 μM AG1296. Phosphorylation of p38 was examined by immunoblotting using a phospho-specific antibody, quantified as the ratios of phospho-p38 and p38, and expressed as percentage of PDGF alone (as noted for BPASMCs in boxes below blots). Bar graphs represent means ± SD for 3 separate experiments in HPASMCs. B: P38 inhibitor SB203580 attenuates PDGF-induced DNA synthesis in a dose-dependent manner. Quiescent HPASMCs and BPASMCs were pretreated with SB203580 (1 or 10 μM) for 30 min and then stimulated with 10 ng/ml PDGF for 24 h. Shown are means ± SD for n = 3. *Significant difference from untreated cells (P < 0.05). #Significant difference from cells treated with PDGF alone at P < 0.05.