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. 2010 Dec 30;300(3):H725–H735. doi: 10.1152/ajpheart.00696.2009

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Fig. 4. — Expression of leukocyte-type 12/15-LO protein and RNA in mouse arteries. A: Western immunoblot for leukocyte-type 12/15-LO (75 kDa) in lysates (30 μg protein/lane) prepared from the mouse aorta and mesenteric arteries with a commercial antibody (Cayman Chemical) and an author-generated (Onsite) antibody. B: RT-PCR products amplified using primers specific for leukocyte-type 12/15-LO (272 bp) and GAPDH (175 bp) probing cDNA prepared from the mouse mesenteric arteries, aorta, lung, and liver.