Table 1.

LC-MS/MS analysis of prostaglandins and HETEs

Sample	Retention Time, min	Major Ion [M-H], m/z	MS/MS Daughter Ions, m/z
6-Keto-PGF1α
Standard	13.2	369	351 ([M-H]-H2O), 333 ([M-H]-2H2O), 315 ([M-H]-3H2O), 289 ([M-H]-2H2O-CO2), 271 ([M-H]-3H2O-CO2), 245, 207, 163*
Biological	13.1	369	351 ([M-H]-H2O), 333 ([M-H]-2H2O), 315 ([M-H]-3H2O), 289 ([M-H]-2H2O-CO2), 271 ([M-H]-3H2O-CO2), 245, 207, 163*
PGE2
Standard	15.5	351	333 ([M-H]-H2O), 315* ([M-H]-2H2O), 271([M-H]-2H2O-CO2), 233 ([M-H]-H2O-C6H120), 189 (233-CO2)
Biological	15.5	351	333 ([M-H]-H2O), 315* ([M-H]-2H2O), 271 ([M-H]-2H2O-CO2), 233 ([M-H]-H2O-C6H120), 189 (233-CO2)
12-HETE
Standard	15.7	319	301 ([M-H]-H2O), 257 ([M-H]-H2O-CO2), 208 ([M-H]-C8H15), 179* ([M-H]-C9H16O), 135 (179-CO2)
Biological	15.7	319	301 ([M-H]-H2O), 257 ([M-H]-H2O-CO2), 208 ([M-H]-C8H15), 179* ([M-H]-C9H16O), 135 (179-CO2)
15-HETE
Standard	14.4	319	301* ([M-H]-H2O), 257 ([M-H]-H2O-CO2), 219 ([M-H]-C6H12O), 175, (219-CO2)
Biological	14.4	319	301* ([M-H]-H2O), 257 ([M-H]-H2O-CO2), 219 ([M-H]-C6H12O), 175, (219-CO2)

LC, liquid chromotography; MS, mass spectroscopy; m/z, mass-to-charge ratio; HETE, hydroxyeicosatetraenoic acid.

^*Daughter ion of greatest abundance.