Table 1.

Summary of experiments

			Challenge		Treatment
Experiment	Rat Strain	Method of Perfusion	Agent*	Concentration, ng/ml	Drug#	Dose	No. of Rats
1. Effect of differing edema toxin doses	Sprague-Dawley	Nonrecirculating	Diluent	0			11
			PA	100			4
			PA	1,000			4
			ETx	50			5
			ETx	100			5
			ETx	200			5
2. Effect of edema toxin inhibition with PA mAb	Sprague-Dawley	Recirculating	PA	100	Placebo		10
			PA	100	PA mAb	10×	4
			Diluent	0	PA mAb	10×	3
			ETx	50	Placebo		6
			ETx	50	PA mAb	10×	5
3. Effect of edema toxin inhibition with adefovir	Sprague-Dawley	Recirculating	PA	100	Diluent		7
			Diluent	0	Adefovir	0.001 μM	6
			Diluent	0	Adefovir	0.01 μM	3
			Diluent	0	Adefovir	0.1 μM	3
			ETx	50	Diluent		10
			ETx	50	Adefovir	0.001 μM	4
			ETx	50	Adefovir	0.01 μM	2
			ETx	50	Adefovir	0.1 μM	2
4. Comparison of the effects of edema toxin and dobutamine	Sprague-Dawley	Nonrecirculating	PA	100			5
			Dobutamine	12.5			6
			ETx	50			5
5. Effect of edema toxin on myocardial and effluent cAMP	Sprague-Dawley	Nonrecirculating	PA	100	Placebo		4
			ETx	50	Placebo		4
			ETx	50	PA mAb	10×	4
			ETx	50	Adefovir	0.001 μM	4
6. Effect of differing LeTx doses	Sprague-Dawley	Nonrecirculating	Diluent	0			11
			PA	100			4
			PA	1,000			4
			LeTx	50			5
			LeTx	500			5
7. Comparison of the effects of LeTx in Sprague-Dawley versus Lewis rats	Spague-Dawley	Nonrecirculating	PA	1,000			3
			LeTx	500			3
			PA	5,000			3
			LeTx	2,500			3
	Lewis	Nonrecirculating	PA	5,000			4
			LeTx	2,500			6

^*ETx, edema toxin; LeTx, lethal toxin; PA, protective antigen;

^#PA mAb, monoclonal antibody against PA.