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. 2011 Mar 7;108(12):5051–5056. doi: 10.1073/pnas.1009516108

Fig. 4.

Fig. 4.

Use of M. smegmatis to identify Mtb genes involved in heme uptake. (A) Mtb and M. smegmatis genomic regions surrounding Rv0203. Rv0203 and M. smegmatis homologs, Msmeg0242 and Msmeg0243, are depicted with solid arrows. The dashed arrows depict genes with high sequence homology between the two genomes. Note that the corresponding genomic region in M. smegmatis contains all of the genes in the genomic region of Mtb surrounding Rv0203. (B) Growth curves of M. smegmatis mc2155 (WT), MsmegΔhemeuptake (genes inclusive of Msmeg0240–Msmeg0251, designated KO) and MsmegΔhemeuptake::Rv0202c-Rv0207c (complemented with Mtb genes inclusive of Rv0202c–Rv0207c, designated KO+Mtb complement) in 7H9–0.01% tyloxapol-NoFe medium supplemented with 1 μM FeCl3, 1 μM heme, or no supplement (No Sup).