Fig. 3.

Chromatin retention in mature sperm distinguishes K81 from HipHop. (A–I) Merged images of K81 foci in spermatids or sperm in WT males expressing various hiphop-k81 chimeric genes tagged with GFP, with DAPI signals in white and GFP signals in red. The names and schematics of the chimeric proteins are shown to the left of picture panels. For name and shading definitions for the chimera, see Fig. 2 and the text. The extent of k81 rescue for each construct is stated under the schematics for the chimeras. In J, DAPI signals from mature sperm are in white. J′ is an overexposed picture of the GFP channel showing no GFP foci on sperm chromatin shown in J.