Fig. 1.

CK2 is necessary for regulation of Maf1 activity by carbon source. Wild-type cells and isogenic mutants cka1-Δ, cka2-Δ, and cka1-Δ cka2^ts were examined by Western blot using anti-Maf1 antibody. Cells were grown to exponential phase in rich glucose medium (YPD Exp) at 30 °C, transferred to glycerol medium (YPGly), incubated at 30 °C for 3 h, then transferred to (A) prewarmed YPD, and incubated at 37 °C (B) prewarmed rich glucose medium containing 4% vol/vol DMSO or 200 μM TBBt, and incubated at 30 °C. Cells were harvested as indicated. Arrows indicate phosphorylated forms of Maf1.