Fig. 2.

Maf1 interacts with CK2 catalytic subunit. YPH499 strain expressing HA-tagged Cka2 and WT control strain were grown in rich glucose medium to exponential phase (YPD), transferred to a nonfermentable glycerol medium (YPGly), and incubated for 3 h. Subsequently, cellular extracts were prepared and subjected to immunoprecipitation with magnetic beads coated with anti-HA antibodies, followed by elution of bound proteins. Total cellular extracts (TOT), flow through (FT), and immunopurified proteins were analyzed by SDS/PAGE and Western blot using anti-HA and anti-Maf1 antibodies. Arrow indicates hyperphosphorylated Maf1 and asterisk indicates hypophosphorylated Maf1.