Fig. 6.

CK2 binds to tRNA in carbon source and Maf1-sensitive manner. Relative occupancy of Cka1-Myc at selected class III genes. Strains WT and maf1-Δ, both expressing Myc-tagged α-catalytic subunit of CK2 (Cka1-Myc) were grown in rich glucose medium (YPD) to exponential phase, transferred to a nonfermentable glycerol medium (YPGly), and incubated at 30 °C for 40 min. Cross-linked chromatin was immunoprecipitated with antibodies against Myc epitope, followed by real-time quantitative PCR. All occupancies are expressed relative to TEL15 region. The experiment was performed in triplicate to estimate SD.