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The progression of acrosomal exocytosis. Capacitated spermatozoa immobilized on a coverslip were incubated with BSA–HTF containing Alexa-594 PNA and thereafter observed under an epifluorescence microscope. Representative time-lapse photographs show a spermatozoon undergoing the acrosomal exocytosis induced by 10 μM ionomycin. Acrosomal EGFP disappearance took place within about 10 s followed by acrosomal staining with PNA lectin.
