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Map-based cloning of the BC14 gene. (A) The bc14 locus was mapped to a 57-kb region on chromosome 2. (B) Structure of the BC14 gene. Lines represent introns and black boxes represent exons. (C) Constructs for complementation tests. (D) A cleaved amplified polymorphic sequence marker (digested with AvaII) used to distinguish between the backgrounds of the wild-type, bc14, and transgenic plants. M, DNA marker.
