Table 1.
Monosaccharide compositional analysis of wall residues from the culms of wild-type and bc14 plants (μg mg−1 AIR)
Residues | Rhamnose | Fucose | Arabinose | Xylose | Mannose | Galactose | Glucose | GalUA | GlcUA | Cellulose |
WT | 3.5 ± 0.0* | 0.9 ± 0.0 | 86.2 ± 1.1* | 280.6 ± 1.8* | 0.4 ± 0.0* | 9.9 ± 0.3* | 45.2 ± 1.1* | 10.1 ± 0.3* | 6.5 ± 0.4* | 374.2 ± 5.6* |
bc14 | 5.4 ± 0.3* | 1.0 ± 0.1 | 157.0 ± 8.0* | 400.4 ± 23.4* | 2.1 ± 0.1* | 15.1 ± 0.6* | 31.0 ± 1.1* | 16.6 ± 0.3* | 11.0 ± 1.2* | 234.9 ± 9.8* |
pBC14F | 2.9 ± 0.1* | 0.8 ± 0.0 | 63.1 ± 0.9* | 249.3 ± 2.1* | 0.4 ± 0.0* | 10.3 ± 0.3* | 74.1 ± 1.6* | 10.8 ± 0.2* | 5.3 ± 0.0* | 361.5 ± 18.7* |
The mounts of monosaccharides are as determined by GC-MS analysis of trimethylsilane derivatives. Cellulose is as determined by Updegraff quantification after TFA hydrolysis.
*Significantly different (t test at P < 0.05) compared with wild type (n = 4) ± SD.